Practical and theoretical consideration of recombinant DNA techniques, including plasmid construction, PCR, mutagenesis, transformation, sequencing, and bioinformatic analysis. The use of recombinant DNA for protein expression. Graded P or F.
Replication and roles of nucleic acids in cellular and viral systems; genome organization, vector-host systems, expression and regulation of genes; catalytic RNA, genetic code evolution, RNA modification and processing, and anti-sense systems.
Principles of biochemical techniques including spectrophotometry, column chromatography, electrophoresis, metabolite assay, enzyme assay, nucleic acid isolation and protein isolation.
Investigation of the properties of enzymes, lipids, carbohydrates and nucleic acids by physicochemical methods and specific assays; purification and quantitation of biochemical compounds by various biochemical techniques.
Credit(s): 2
Corequisite(s):
(for BCB and BISC students) LANG 3014 AND LIFS 2820; (for others) LIFS 2820
Major classes of biochemical compounds; primary, secondary, tertiary and quaternary structures of macromolecules; enzyme kinetics and mechanisms; biosynthesis of DNA and RNA; transfer RNA and protein synthesis.
Advancement in science has enabled scientists to modify biological systems for specific purposes. Many of these technologies have been used to improve our daily life and this area of study is commonly referred to as biotechnology. This course is designed to introduce some of the major subjects in this field including the history of biotechnology, ethics in genetic modification and use of animals in experimental studies, molecular foundation of biotechnology, animal biotechnology, plant and agricultural biotechnology, and health-care applications. You will learn how different aspects of biotechnology affect our daily life and have impact on the society.
Diversity of life; taxonomy and phylogeny; different kingdoms of living organisms; structure and function; evolution and the generation of biodiversity.
UPDATE: The course will be offered in Winter and Spring 2021-2022 (a two-semester course)
This course engages students in practical training and enriches their knowledge in biodiversity and skills in interpretive services under ecotourism. The students will also have a chance to contribute to the society through environmental education. At the end of the course, students should gain hands-on experience in environmental conservation and improve their skills in communication, problem solving, team work, and time management. For LIFS, ENVS and OST students only. Approval of the course coordinator is needed for students to enroll in this course. Graded PP, P or F.
Our current understanding of biochemical reactions and internal organization of a cell was progressively enriched by technological and conceptual breakthroughs in the 20th century. This course will use personal and scientific stories of six prominent scientists to highlight research areas that are still being actively pursued today. Topics to include are DNA and protein biochemistry, physical and chemical methods for biological research, and the use of model organisms.
