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Wan-Keung R. WONG

Wan-Keung R. WONG
APPOINTMENT
Associate Professor Emeritus
TITLE
PhD The University of British Columbia

CONTACT

bcwkrw@ust.hk

23587299

PUBLICATIONS

HKUST Scholarly

Research Question

Bacterial genetics; cloning and expression of genes in Escherichia coli and Saccharomyces cerevisiae; excretion of proteins from E. coli; development of E. coli systems for extracellular production of valuable proteins; production and reconstitution of recombinant cellulases for use in energy generation and cellulosic waste management; investigation and application of Bacillus subtilis as a recombinant host for heterologous gene expression.

Representative Publications

  1. Wong, W.K.R., Ng, K.L. and Lai, C.Y. (2021) Scar-free healing of wounds resulting commonly from domestic accidents. Medical & Clinical Research, 6:(4): 531-535.
  2. Siddique, F., Lam, C.C., Ng, K.L., Chan, A.K.N. and Wong, W.K.R. (2021) Characterization and expression of a cellobiase Cba2 of Cellulomonas biazotea: the first bacterial β-glucosidase emonstrated to possess a genuine signal peptide. Research Journal of Biotechnology, 16(6):1-14.
  3. Lai, C.Y., Ng, K.L., Wang, H., Lam, C.C. and Wong, W.K.R. (2021) Spontaneous cleavages of a heterologous protein, the CenA endoglucanase of Cellulomonas fimi, in Escherichia coli. Microbiology Insights, 14:1-10.
  4. Wong, W.K.R., Ng, K.L. and Lai, C.Y. (2021) Application of recombinant skin growth factors to effective treatment of bedsores. Journal of Biomedical and Biological Sciences, 1(1):1-9.
  5. Tsang, M.W., Wong, W.K.R., Hung, C.S., Lai, K.M., Tang, W., Cheung, E.Y.N., Kam, G., Leung. L., Chan, C.W., Chu, C.M. and Lam, E.K.H. (2003). Human epidermal growth factor enhances healing of diabetic foot ulcers. Diabetes Care 26: 1856-61.

Awards / Honors

 

2018 Asian Chinese Leader, to be awarded by Asian College of Knowledge Management, 21 July 2018, Hong Kong
2018 Fellowship, awarded by Social Enterprise Research Academy, 5 May 2018, Hong Kong
2017 Invited as a keynote speaker in the “International Conference on Genetics & Molecular Research”, May 22-24, 2017 at Dubai, UAE.
2016 Nominated by the Europe Business Assembly (EBA, Oxford, Great Britain) to be recipient of the International award “Best Enterprise”
2016 Golden Medical Award, awarded by Global Medical Discovery. Canada
2015 Nominated by the Europe Business Assembly (EBA, Oxford, Great Britain) to be recipient of the International award “Best Manager of the Year”
2015 “Certificate of Recognition” presented by the “International Conference on Targeting Diabetes and Novel Therapeutics” held on September 14-16 in Las Vegas, Nevada, USA
2015 Most Innovative Biotechnology Group, awarded by Hong Kong Mediazone.
2015 Most Valuable Services Awards in Hong Kong 2015, awarded by Hong Kong Mediazone.
2014 Higher Education Outstanding Scientific Research Output Award, Nominated by HKUST
2014 全球80位傑出華人企業家管理韜略與人文哲思，華商領軍者, awarded by 中國日報( China Daily) , 中國經濟出版社。
2013 香港傑出商社領袖人物, awarded by 文匯報 15 March 2013
2012 Named as one of the “中華愛國英才” and a 4-page introduction was published in 〈中華愛國國典〉2012
2012 Invited by the China Economic Trading Promotion Agency (CETPA) to be its representative in the biomedical sector to present at the 6th World Chinese Entrepreneurs Forum in Bangkok, 3 May 2012
2012 2012 Most Respected Chinese Entrepreneur in Asia Pacific, awarded by the China Economic Trading Promotion Agency (CETPA)
2011 2011 Most Promising Entrepreneurship Award Winner of the prestigious Asia Pacific Entrepreneurship Awards, Hong Kong
2008 2008 Hong Kong Awards for Industries: Technological Achievement Certificate of Merit. Hong Kong Science and Technology Parks

II.Research

II.a. Publications
II.a.(i). Journal Papers
  1. Wong, W.K.R., Ng, K.L. and Lai, C.Y. (2021) Scar-free healing of wounds resulting commonly from domestic accidents. Medical & Clinical Research, 6:(4): 531-535.
  2. Siddique, F., Lam, C.C., Ng, K.L., Chan, A.K.N. and Wong, W.K.R. (2021) Characterization and expression of a cellobiase Cba2 of Cellulomonas biazotea: the first bacterial β-glucosidase emonstrated to possess a genuine signal peptide. Research Journal of Biotechnology, 16(6):1-14.
  3. Lai, C.Y., Ng, K.L., Wang, H., Lam, C.C. and Wong, W.K.R. (2021) Spontaneous cleavages of a heterologous protein, the CenA endoglucanase of Cellulomonas fimi, in Escherichia coli. Microbiology Insights, 14:1-10.
  4. Wong, W.K.R., Ng, K.L. and Lai, C.Y. (2021) Application of recombinant skin growth factors to effective treatment of bedsores. Journal of Biomedical and Biological Sciences, 1(1):1-9.
  5. Hu, X.H., Lai, C.Y.N., Sivakumar, T., Wang, H., Ng, K.L., Lam, C.C. and Wong, W.K.R. (2018) Novel Strategy for expression of authentic and bioactive human basic fibroblast growth factor in Bacillus subtilis. Appl Microbiol Biotechnol. 102(16): 7061-7069. DOI 10.1007/s00253-018-9176-1
  6. Sivakumar, T., Lai, C.Y.N., Lam, C.C., Hu, X.H., Wang, H., Ng, K.L. and Wong, W.K.R. (2018) Purification of authentic human basic fibroblast growth factor expressed in both the cytoplasm and culture medium of Escherichia coli. International Journal of Chromatography and Separation Techniques. IJCST-115. DOI: 10.29011/2577-218X. 000015
  7. Chan, K.N. A., Ng, K.L.A., Ng, K.Y.K. and Wong, W.K.R. (2018) Cloning and characterization of two novel beta-glucosidase genes encoding isoenzymes of the cellobiase complex from Cellulomonas biazotea. Gene. 642: 367-375.
  8. Wong, W.K.R., Ng, K. L., Lam, C.C., Hu, X.H., Lai, N.C.Y., Wang, H., and Sivakumar, T. (2017) Review Article: Reasons for Underrating the Potential of Human Epidermal Growth Factor in Medical Applications. J Anal Pharm Res 4(2): 00101. DOI: 10.15406/japlr.2017.04.00101
  9. Kwong, K. W. Y., T. Sivakumar, Wong, W.K.R. (2016) Intein mediated hyper-production of authentic human basic fibroblast growth factor in Escherichia coli. Scientific Reports. 6:33948; doi: 10.1038/srep33948.
  10. Wong, W.K.R., Kwong, K. W. Y., Ng, K. L. (2016) Application of Recombinant Human Epidermal Growth Factor to Effective Treatment of Scalds. J Anal Pharm Res 3(1): 00045. DOI: 10.15406/japlr.2016.03.00045.
  11. Kwong, K. W. Y., Ng, K. L., Wong, W.K.R. (2016) Engineering versatile protein expression systems mediated by inteins in Escherichia coli. Appl Microbiol Biotechnol. 100:255-262.
  12. Ng, A.K.L., Lam, G.C.C., Kwong, K.W. Y., Dik, D.W.N., Lai, N.C.Y., Yong, J.L.W., Qian, P.Y., Wong, W.K.R. (2016) Enhancement of fish growth employing feed supplemented with recombinant fish growth hormone expressed in Bacillus subtilis. Research Journal of Biotechnology. 11(1): 1-11.
  13. Wong, W.K.R. (2015) Effective Treatment of hard-to-heal surgical wound with recombinant human epidermal growth factor. Modern Chemistry & Applications. 3(4):166, 1-3.
  14. Wong, W.K.R. (2015) Recombinant production of human skin growth factor for effective treatments of hard-to-heal wounds. J. Diabetes Metab.6 (8): 52.
  15. Wong, W.K.R. (May 2014) Means and methods for producing authentic human basic fibroblast growth factor. United States Patent Application, Pub. No.: 2014/0134673 A1.
  16. Kwong, K. W. Y., Wong, W.K.R. (2013) A revolutionary approach facilitating co-expression of authentic human epidermal growth factor and basic fibroblast growth factor in both the cytoplasm and culture medium of Escherichia coli Appl Microbiol Biotechnol. 97:9071-9080.
  17. Kwong, K. W.Y., Ng, A.K.L., Lam, C.C., Wang, Y.Y., Wong, W.K.R. (2013) Authentic human basic fibroblast growth factor produced by secretion in Bacillus subtilis. Appl Microbiol Biotechnol. 97:6803-6811.
  18. Wong, W.K.R., Fu, B.Z.B., Wang, Y.Y., Ng, A.K.L. and Chan, A.K.N. (2012) Engineering of efficient Escherichia coli excretion systems for the production of heterologous proteins for commercial applications. Recent Patents on Chemical Engineering 5:45:55 (Invited Review).
  19. Chan, A.K.N., Wang, Y.Y., Ng, A.K.L., Fu, B.Z.B. and Wong, W.K.R. (2012) Cloning and characterization of a novel cellobiase gene, cba3, encoding the first known β-glucosidase of glycoside hydrolase family 1 of Cellulomonas biazotea. Gene 493:52-61.
  20. Wang, Y.Y., Fu, B.Z.B., Ng, A.K.L., Lam, G.C.C., Chan, A.K.N., Sze, B.K.F. and Wong, W.K.R. (2011) Enhancement of excretory production of an exoglucanase from Escherichia coli with phage shock protein A (PspA) over-expression. J Microbiol Biotechnol. 21:637-645.
  21. Wang, Y.Y., Ng, A.K.L., Lam, G.C.C., Chan, A.K.N., Sze, B.K.F., Fu, B.Z.B. and Wong, W.K.R. (2010) Efficient Bacillus subtilis promoters for graded expression of heterologous genes in Escherichia coliRes. J. Biotech.5:5-14.
  22. Wong, W.K.R. (2008) Application of recombinant microbial systems to the production of commercially valuable proteins. Res. J. Biotech. 3 (3): 3-4.
  23. Ng. K.L., Lam, C.C., Fu, Z.B., Han, Y.F., Tsim, K.W.K. and Wong, W.K.R. (2007) Cloning and characterization of the yjeA gene, encoding a novel deoxyribonuclease, from Bacillus subtilis. J. Biochem. 142:647-654.
  24. Fu, Z.B. and Ng, K.L., Lam, C.C., Leung, K.C., Yip, W.H. and Wong, W.K.R. (2006) A two-stage refinement approach for the enhancement of excretory production of an exoglucanase from Eshcerichia coli. Protein Expression and Purification. Protein Expression and Purification 48:205-214.
  25. Chen, X., Xu, Z., Cen, P. and Wong, W.K.R. (2006) Enhanced plasmid stability and production of hEGF by immobilized recombinant E. coli JM101. Biochemical Engineering Journal 28:315-219.
  26. Fu, Z.B., Lam, T.L. and Wong, W.K.R. (2005) Cell death caused by “saturated translocation” of a secretory exoglucanase in Escherichia coli. Protein Expression and Purification 42:67-77.
  27. Chen, X., Xu, Z., Cen, P. and Wong, W.K.R. (2005) A novel two-stage continuous process for excretive expression of hEGF by recombinant E. coli JM101. Process Biochemistry 40:1-4.
  28. Tsang, M.W., Tsang, K.Y. and Wong, W.K.R. (2004) A case report on the use of recombinant human epidermal growth factor (rhEGF) in a gentlemen with drug induced Steven Johnson Syndrome. Derm. Ontline J. 10 (1): Article 25.
  29. Tsang, M.W., Wong, W.K.R., Hung, C.S., Lai, K.M., Tang, W., Cheung, E.Y.N., Kam, G., Leung. L., Chan, C.W., Chu, C.M. and Lam, E.K.H. (2003). Human epidermal growth factor enhances healing of diabetic foot ulcers. Diabetes Care 26: 1856-61.
II.a.(ii). Articles
  1. 黃允強, 序言, 跟蹤.鍾根, 新民主出版社有限公司 2016
  2. 黃允強, 議員執靚Look勇戰立會 , East Week · 663, page 04, 11th May 2016
  3. 黃允強 (December 2014) Promotional description of Gene-vinate Limited. The People’s Republic of China Yearbook 2014, 2014 中華人民共和國年鑑.
  4. 黃允強 (March 2014) 經營美麗事業 指引健康之路, 全球80位傑出華人企業家管理韜略與人文哲思， 華商領軍者, 中國日報( China Daily) , 中國經濟出版社。
  5. Wong, W.K.R. (2014) Entrepreneur special: Spreading fruits of science- CEO brings skin treatment within reach of consumers (Translated by Zhuan Ti). China Daily 9th Feb 2014.
II.b. Patents
Patents
  1. Wong, W.K.R., Lam C.C., Ng, K.L. (2018) Novel Means and Method for production of hbFGF with Bacillus subtilis. Hong Kong Short-term No. 18105835.8; filed on 7 May 2018.
  2. Wong, W.K.R., Kwong, W. Y. (2017) Improved means and method for hyper-production of authentic human basic fibroblast growth factor in Escherichia coli. Hong Kong short-term patent No. HK1228651; Granted on November 3, 2017.
  3. Wong, W.K.R. (2017) Means and method for expression of authentic human epidermal growth factor and/or basic fibroblast growth factor in cytoplasm and/or culture medium of Escherichia coli Hong Kong short-term patent No. HK1219200; Granted on March 24, 2017
  4. Wong, W.K.R. (2017) Means and method for expression of authentic human epidermal growth factor and/or basic fibroblast growth factor in cytoplasm and/or culture medium of Escherichia coli US Patent No. 9,580,484 B2; Granted on February 28, 2017.
  5. Wong, W.K.R., Kwong, W. Y. (2016) Improved means and method for hyper-production of authentic hbFGF in E. coli. Hong Kong Patent Application submitted.
  6. Wong, W.K.R., Ng, K. L. (2015) Means and method for enhancing fish growth. Hong Kong Patent Application No.: 15112136.3; filed on December 09, 2015
  7. Wong, W.K.R. (2015, pending) Means and method for expression of authentic human epidermal growth factor and/or basic fibroblast growth factor in cytoplasm and/or culture medium of Escherichia coli US Provisional Patent Application No.: 61/808.062; filed on 4 March 2013.
  8. Wong, W.K.R. (2015) Means and methods for producing authentic human basic fibroblast growth factor. US Patent No.: 9,017,968 B2; Granted on 28 April 2015.
  9. Wong, W.K.R., Lam, K.H.E. and Tsang, M.W. (2014) Method and composition for treating skin wounds with epidermal growth factor. German Patent 60346186.7; Granted on 18 Jun 2014.
  10. Wong, W.K.R., Lam, K.H.E. and Tsang, M.W. (2014) Method and composition for treating skin wounds with epidermal growth factor. PCT European Patent EP1482966B1; Granted on 14 May 2014.
  11. Wong, W.K.R., Lam, K.H.E. and Tsang, M.W. (2011) Method and composition for treating skin wounds with epidermal growth factor. Taiwan PCT National Phase Patent, Taiwanese Patent No.: I 348912. Granted on 11 Sep 2011
  12. Wong, W.K.R., Lam, K.H.E. and Tsang, M.W. (2009) Method and composition for treating skin wounds with epidermal growth factor. China PCT National Phase Patent, Chinese Patent No. ZL03809985.3. Granted on 23 Dec 2009
  13. Wong, W.K.R., Lam, K.H.E. and Tsang, M.W. (2009) Method and composition for treating skin wounds with epidermal growth factor. United States PCT National Phase Patent, US Patent No. 7,517,528 B2. Granted on 14 Apr 2009
II.c. Invited Lectures
  1. “Factors deferring the application of skin growth factors as drugs to the treatments of wounds”. Invited as a keynote speaker in the “International Conference on Genetics & Molecular Research” to be held during May 22-24, 2017 at Dubai, UAE.
  2. “Engineering Efficient Microbial Systems for Recombinant Production of Useful Proteins”, 2016 International Conference on Animal Vaccine and Adjuvant Technology. National Pingtung University of Science and Technology, Taiwan, 31st May 2016
  3. “Efficient microbial systems for protein expression “, Malaysia Innovation Hub, Ministry of Education Malaysia, Malaysia, 22nd October 2015
  4. “Efficient microbial systems for protein expression “, Faculty of medicine, University of Malaya, Malaysia, 21st October 2015
  5. “Efficient microbial systems for protein expression “, Universtiti Teknologi Malaysia, Malaysia, 20th October 2015
  6. “Efficient microbial systems for protein expression and vaccine development“, International conference on animal vaccine and adjuvant technology, Graduate Institute of Animal Vaccine Technology, National Pingtung University of Science and Technology, National Pingtung University of Science and Technology, Taiwan, 6th Oct 2015
  7. “Medical application of recombinant hEGF “, Diabetic Medications 2015, Las Vegas, 14th – 16th, September 2015
  8. “Tremendous Potential in Skin Beauty and Care”, BIOtech Japan 2015- 14th International Bio Technology Exhibition & Conference, 13th-15th May, 2015